Eppendorf Centrifuge 5414 Manual -
Without the original manual, many users attempt to run the 5414 at maximum speed for every protocol. A direct quote from the manual: “For most DNA precipitation and cell debris pelleting, 8,000–10,000 rpm is sufficient. Maximum speed should be reserved for bacterial pellets and phase separation.”
to prevent imbalance. Opposite tubes must be of the same type and contain equal mass. Setting Time eppendorf centrifuge 5414 manual
is a table-top centrifuge commonly used with fixed-angle rotors for 1.5/2.0 mL tubes. Without the original manual, many users attempt to