Imagej Image Comparison [PROVEN | BLUEPRINT]

PCC measures the linear relationship between pixel intensities across two images. A value of +1 means identical, 0 means no correlation, and -1 means inverse correlation.

Need to prove a change in brightness or density? Don't use subtraction. imagej image comparison

Now, open your images, run the Image Calculator, and let the pixels tell you the truth. 0 means no correlation